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. 2020 Jan 31;10:1558. doi: 10.1038/s41598-020-57588-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The PI3K/AKT pathway genes expressions were changed by overexpression of miR-548x or miR-4698. The qRT-PCR was used to investigate gene expression in A-172 (A) and U251 (B) cell line after 72 h of miR-548x or miR-4698 overexpression compared with the respective control. Data are presented as mean ± SD of results from 3 independent investigations, (^∗P < 0.001) as compared with the scramble. Results were normalized using B2M as an internal reference gene.