Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jan 31;10:1557. doi: 10.1038/s41598-020-57994-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Different stresses can inhibit ataxin-1 NB exchange dynamics either irreversibly or reversibly. Neuro-2a cells were transfected to express GFP-ataxin-1[85Q]. At 24 h post-transfection, cells were left untreated (Ctrl) or treated with arsenite (0.3 mM, 1 h) (Arsenite) or sorbitol (0.5 M, 1 h) (Sorbitol). Following wash out (three washes with PBS), cells were left to recover from the stress treatment for 3 h (Arsenite wash out or Sorbitol wash out). (A) Representative images are shown from 3 independent experiments for quantitative assessments of GFP-ataxin-1[85Q] exchange dynamics. White rectangle indicates ataxin-1 NB imaged analyzed. A small ROI (indicated by white open arrowhead for dynamic NB or white solid arrowhead for non-dynamic NB) was photobleached and the fluorescence recovery was subsequently monitored as post-bleach at 5 s intervals for 250 s. (B) Plot of the percentage recovery of fluorescence from experiments as shown in (A). Each symbol represents fluorescence measured at the indicated time. (C,D) Results for pooled data for FRAP experiments. The recovery of fluorescence for GFP-ataxin-1[85Q] over time was analyzed. Each symbol represents a single data point. Results represent the mean ± SEM for (C) fluorescence maximum recovery percentage in the bleached area (where 100% represents full recovery) and (D) the initial rate of fluorescence recovery percentage in the bleached area for first three time points (0–15 s of post-bleach). Significance values were calculated by (C) ANOVA or (D) Mann-Whitney and Kruskal-Wallis non-parametric test, n > 7, ****p < 0.0001, ^###p < 0.001, n.s. = not significant. All scale bars = 10 μm.