Figure 5.

Liquid-like or solid-like properties of ataxin-1 NBs under control or stress conditions. Neuro-2a cells were transfected to express GFP-ataxin-1[85Q]. At 24 h post-transfection, cells were left untreated (Ctrl) or treated with arsenite (0.3 mM, 1 h) (Arsenite) or sorbitol (0.5 M, 1 h) (Sorbitol). Cells were further treated with 1,6-hexanediol (5%, 5 min) to discriminate between liquid-like assemblies and solid-like assemblies. Cells were then fixed and stained with DAPI before CLSM imaging. Each symbol represents a single data point. Representative images are shown from 3 independent experiments. All scale bars = 10 μm.