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Kinetic parameters obtained from the progress curve analysis for the hydrolysis of substrates 1 and 2 promoted by MuV-HN.
| KM (µM) |
Vmax (mM/min) | Kcat (min−1) |
Kcat/KM (mM−1min−1) | |
|---|---|---|---|---|
| trisaccharide 1 | 12 | 0.0007 | 0.1 | 8.55 |
| undecasaccharide 2 | 3.5 | 0.000023 | 0.0025 | 0.65 |
