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. 2020 Jan 31;10:1614. doi: 10.1038/s41598-020-58667-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Plasma-activated medium (PAM) induces autophagy of endometrial cancer cells. (A) Representative images of Immunocytochemistry staining by LC3B with or without PAM treatment for AMEC cells. (B) Expression levels of LC3 protein after 0.5–6 h PAM treatment in AMEC cells were evaluated by Western blotting in different concentrations. (C,D) Effect of PAM on mTOR and AKT activation after 0.5–6 h in different PAM concentrations in AMEC (C) and HEC50 (D) cells. (E,F) Expression levels of LC3, p62 and ATG family proteins after 1:4 PAM treatment in AMEC (E) and HEC50 (F) cells at 0–24 h. The numbers indicated the density value normalized to GAPDH using the ImageJ software. The density value of phosphorylated form was expressed as the ratio to the density value of total protein. The original blots are presented in Supplementary Fig. 3.