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. 2020 Jan 31;10:1563. doi: 10.1038/s41598-020-58502-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Scheme of the treatment of HPV-negative (FaDu) and HPV-positive (2A3) cells and tumors with concurrent cisplatin (CDDP) and a single-dose or fractionated irradiation regimen. (a) Time course of cell treatment with concurrent CDDP and a single-dose irradiation (2 Gy, IR) on day 2 for flow cytometry analyses. (b) Time course of cell treatment with concurrent CDDP fractionated irradiation regimen (3 × 2 Gy, FIR) for flow cytometry analyses. (c) Time course of tumor treatment with concurrent CDDP and IR (10 Gy) (d) Time course of tumor treatment with concurrent CDDP and FIR (8 × 2 Gy). CDDP was injected intravenously 20 min prior to single-dose or fractionated irradiation regimen on the first day only.