Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jan 31;11:645. doi: 10.1038/s41467-019-14164-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a Green fluorescence (for KFERQ-Dendra) and immunofluorescence (for LAMP1) of cerebral cortex from KFERQ-Dendra mice. Insets: boxed area at higher magnification. Nuclei are highlighted in blue. b, c Green KFERQ-Dendra fluorescence in the same regions immunostained with MAP2 (to label neurons) (b), GFAP (to label astrocytes) or IBA1 (to label microglia). Arrows: green fluorescent puncta. Boxed areas are shown at higher magnification at the bottom (c). d Green KFERQ-Dendra fluorescence in the indicated regions of the hippocampus of KFERQ-Dendra mice. Boxed areas are shown at higher magnification on the right. The cartoon depicts the areas analyzed and abundance of the fluorescent puncta in each region. e Direct KFERQ-Dendra fluorescence in primary neuronal cultures prepared from KFERQ-Dendra mice brains. Insets show the boxed regions of soma and neurites at higher magnification for the green channel only to better appreciate presence of fluorescent puncta. f Green fluorescence (for KFERQ-Dendra) and immunofluorescence (for GFAP or LAMP1) in primary astrocyte cultures prepared from KFERQ-Dendra mice brains. Boxed areas are shown at higher magnification at the bottom. g Green fluorescence (for KFERQ-Dendra) and immunofluorescence (for LAMP1) in brain sections from KFERQ-Dendra mice 4 days after culture. Merged images are shown on the right, where nuclei are highlighted with DAPI. Boxed areas are shown at higher magnification on the right. Experiments were repeated 3 times (for a, b, d) and 5 times (for c, e, f, g) with similar results.