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. 2020 Jan 31;11:645. doi: 10.1038/s41467-019-14164-4

Fig. 5. Monitoring chemical activation of CMA in vivo.

Fig. 5

a, b Livers from KFERQ-Dendra mice injected with vehicle or acetaminophen (to induce hepatotoxicity) were stained with H&E (a) or imaged for direct fluorescence (for KFERQ-Dendra) or immunostained (for LAMP1) (b). Boxed regions are shown at higher magnification on the right. Right: quantification of the number of puncta per cell. Similar results were obtained in three independent experiments for each condition for a and b. Data points indicate average values from different sections of three independent experiments (n = 8 sections). c–e. KFERQ-Dendra mice were i.p. injected with the CMA activator or vehicle for 3 days and different organs imaged for direct fluorescence (for KFERQ-Dendra) and immunofluorescence for LAMP1 in livers (c) and for MAP2 in brain cortex (d) and direct fluorescence in T cells (e) isolated from blood of the treated animals. Quantification of the average number of puncta per cell is shown on the right. Experiments were repeated three times independently for (c–e). Data points indicate average values from different sections of three independent experiments for each condition n = 9 sections for (c), n = 7 sections for (d), n = 6 sections for (e). Two-tailed unpaired t-tests were used.Source data are provided as a Source Data file.