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. 2019 Dec 13;14(1):127–137. doi: 10.1007/s12072-019-10007-y

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© The Author(s) 2019

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Immunofluorescence detection of AAT and AAT polymers. a Representative pictures of liver organoids in the expansion medium (EM) of MM, MZ and ZZ patients (images magnification ×10). b Differentiated liver organoids from MM, MZ and ZZ patients. Specific detection of total AAT protein with anti-AAT-B9 or with anti-AAT-D11 against AAT polymers, are shown in green fluorescence. Zoomed images of individual positive cells are showed in the right corner. c Quantification of AAT polymers (D11) positive cells in the different organoids MM, MZ and ZZ. Microscope Zeiss Ax10, camera Axio Cam Mrm Carl Zeiss and software AxioVision Rel.4.7 (images magnification ×40)