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. 2020 Jan 31;11:657. doi: 10.1038/s41467-020-14434-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Through micro-3D-printing we fabricate microfluidic devices of a few 100 µm in size. Magnified view of a nozzle placed on a 1 cent coin for scale reference. b This technique can realize integrated nozzle assemblies to jet protein crystal suspension into the X-ray interaction region for diffractive imaging.