Figure 6.

The effect of preconditioning with HIF-PHD inhibitors followed by 24 hours re-oxygenation and 6 hours OGD on PC12 cells. PC12 cells were exposed to the PHD inhibitors (1, 10, 50 and 100 µM) for 24 hours in normoxia (21% O₂) followed by 24 hours re-oxygenation (21% O₂) and subsequent 6 hours OGD insult (0.3% O₂). (A) MTT assay (n = 3) showed greater mitochondrial activity after 6 hours OGD in cells preconditioned with 100 µM of DMOG, FG2216, or GSK1278863 and 50 & 100 µM of FG4592 or Bay 85-3934 compared to 1% DMSO pretreatment; (B) LDH assays (n = 3) revealed reduced LDH release in cells preconditioned with 100 µM of DMOG, FG2216, FG4592, GSK1278863, or Bay 85-3934 prior to 6 hours OGD compared to 1% DMSO pretreatment; (C) Trypan blue exclusion assays (n = 3) reveal increased live (unstained) cells in cells preconditioned with 100 µM of DMOG, FG2216, FG4592, GSK1278863, or Bay 85-3934 prior to 6 hours OGD compared to 1% DMSO pretreatment. Data were expressed as mean ± S.D. *Indicated P < 0.05 against 1% DMSO (Two-way ANOVA, Tukey’s post-hoc analysis).