Fig. 1. Overexpression of At3g62960 (CEPDL2) highly induces the expression of NRT2.1.

a NRT2.1 expression levels in the 10-day-old wild-type (WT) plants or transgenic plants overexpressing At2g30540 (CEPDL1) or At3g62960 (CEPDL2) under N-replete (10 mM NH₄⁺, 10 mM NO₃^–) or 10 mM NO₃^– conditions. For each transgene, results from two independent T2 transgenic lines are shown (mean ± SD, *P < 0.05 by two-tailed non-paired Student’s t test, n = 3). Source data are provided as a Source Data file. b Histochemical staining of 10-day-old seedlings transformed with the CEPDL2pro:GUS gene. Scale bar = 1 mm. c Cross-section of the leaf vascular tissues pictured in b. Xy xylem, Ph phloem. Scale bar = 10 µm. d qRT-PCR analysis of CEPDL2 transcripts in the shoots and roots of 7-day-old WT plants cultured on 10 mM NO₃^– condition (n = 3).