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. 2020 Jan 31;6:5. doi: 10.1038/s41522-020-0115-4

Fig. 6. ΔrelΔrshB biofilms comprising more bacterial cells and protein(s) than biofilms of parent strain 381.

Fig. 6

Parent strain 381 and ΔrelΔrshB mutant were grown for 48 h on glass, and stained with SYTO9 (green color for cells) and SYPRO Ruby (red color for extracellular protein), and three-dimensional z stacks were acquired by fluorescence microscopy. A complete z stack (17 images per stack) was collected using Nikon imaging software (NIS-elements AR 4.30.02, 64-bit). A graticule size: 50 µm (width: 277.41 µm, height: 234.07 µm, and depth: 8 µm). The images shown are representative of three independent experiments. SYTO9, SYPRO Ruby, and total biomass were quantified using Comstat2. Error bars represent the standard deviation. The data were analyzed using the Student’s t test. *p < 0.05.