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. 2020 Jan 31;10:1588. doi: 10.1038/s41598-020-58579-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The cytotoxic effects of (a) menadione, (b) curcumin depend on the time point of drug addition in SiHa and (c) menadione, (d) curcumin depends on the time point of drug addition in HCT116. The cells were seeded at 1 × 10⁴ cells per well in 96 well plates in serum free DMEM. Medium change to DMEM with 10% serum, 24 h post seeding is considered to be 0 h. The drugs were introduced at 0, 4 or 8 h post medium change. MTT assay was done 48 h post drug treatment. Data represented as mean ± SD, n = 3.