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. 2020 Jan 31;5:10. doi: 10.1038/s41541-020-0160-2

Fig. 9. MSP1-specific recall T-cell response.

Fig. 9

PBMCs from six volunteers immunized three times with SumayaVac-1 were exposed to purified MSP1-D (5 µg ml−1 for 24 h) and the recall T-cell response as defined by IFN-γ production was measured in the cultured ELISpot assay. a Representative images of ELISpot wells are shown for different vaccinees. b The net mean spot-forming units (SFU) per 1 million PBMCs were calculated and analyzed as a function of time post the first immunization. Each data point represents the mean from three determinations ± SD. The red arrows indicate the days of immunization with SumayaVac-1. Significance was assessed using Kruskal–Wallis one way ANOVA on ranks. c Stimulation of PBMCs by peptides corresponding to HLA-A0201-restricted MSP1 CD8+ T-cell epitopes. PBMCs from the same vaccinees as above were analyzed (same color code as above), in addition to PBMCs from three semi-immune adults from Burkina Faso (B.F.). The following peptides were used: 291, GLHHLITEL; 374, SLLTELQQV; 437, VIYLKPLAGV; 674, KLKEFIPKV.40,68,69 Statistical significance was assessed using the two tailed t-test.