Fig. 3. Analysis of genetic knockouts and their effect on glycosaminoglycan metabolism.
a Growth of genetic knockouts on glycosaminoglycan substrates (CS-A chondroitin sulfate A, DS dermatan sulfate, CS-C chondroitin sulfate C and HA hyaluronic acid); b TLC analysis of purified oligosaccharide (X), left behind in media from Δbt3324 growths, against PULCS/DS/HA encoded lyases; c HPLC analysis of X treated with BT3324PL8. Data represent technical triplicates and source data are provided in the source data file labelled as mutant growths.