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. 2020 Jan 31;11:646. doi: 10.1038/s41467-020-14509-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Cartoon representation of BT3349-S1_27^4S-sulf ramped blue to red from its N- to C-terminus; b stick representation of binding site interactions with the protein in green and the bound trisaccharide yellow; c surface representation of BT3349-S1_27^4S-sulf showing how the +1 and −1 ends of the trisaccharide are free to be extended; d overlays of surface representations of BT3349-S1_27^4S-sulf and a cartoon of the marine endo 4S ι-carrageenan sulfatase (PsS1_19A). CS and ι-carrageenan oligosaccharides are shown in yellow and cyan, respectively. e Overlay of a surface of BT3349-S1_27^4S-sulf with a cartoon of the exo-sulfatase BT1596-S1_9^2S-sulf. f Overlay of a surface of BT3349-S1_27^4S-sulf with a cartoon of the exo-sulfatase BT3333-S1_15^6S-sulf. g Overlay of a surface of BT3349-S1_27^4S-sulf with a cartoon of the human exo-sulfatase 1FSU^4S-sulf. h Overlay of a surface of BT3349-S1_27^4S-sulf with a cartoon of the human exo-sulfatase 5FQL^2S-sulf. All cartoon representations are ramped blue to red from their N- to C-terminus.