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. 2020 Jan 31;10:1617. doi: 10.1038/s41598-020-58526-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ZnO NP exposure results in ROS (superoxide) generation in B. mori hemocytes. After exposing larvae with ZnO NPs for different time periods, hemocytes were collected and stained with DHE for measurement of superoxide (O₂⁻²) by flow cytometry. Each experiment was performed at least in triplicate. The larvae were pre-treated with ROS inhibitors (5 mM and 10 mM NAC), and then treated with the desired dose of nanoparticles. *P < 0.05, **P < 0.01, ***P < 0.001 versus control. ^###P < 0.001 versus 24 hour group.