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. 2020 Jan 31;10:1617. doi: 10.1038/s41598-020-58526-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Determination of induction of apoptosis in hemocytes by flow cytometric analysis using Annexin V-PI staining following ZnO NP exposure. (a–d) are the representatives of experimental results of one group. Control (a), exposed to ZnO NP (b), treated with ZnO NPs + 5 mM N-acetyl-L-cysteine (NAC) (c) and treated with ZnO NPs + 10 mM N-acetyl-L-cysteine (NAC) (d). The graph (e) represents the results of the whole experiment (3 replicates), the statistical analysis of which was done by One-way ANOVA test, followed by Tukey’s multiple comparison test. The results in control and vehicle-treated larvae were similar (data not given). *P < 0.05, **P < 0.01, ***P < 0.001 versus control. ^#P < 0.05, ^##P < 0.01 versus 24 hour group. ^$P < 0.05 versus ZnO NPs + 5 mM (NAC).