Table 1.
Accumulation/Distribution of Zinc Oxide nanoparticles in various tissues.
| Time\Tissue | μg/g body weight (or μg/ml in case of hemolymph) | |||||
|---|---|---|---|---|---|---|
| Whole Insect | Gut | Hemolymph | Haemocytes | Cuticle | Excreta | |
| 0 hours | * | # | # | # | # | # |
| 6 hours | 4.064 ± 0.033 | 1.100 ± 0.083 | 0.850 ± 0.057 | 0.110 ± 0.005 | 0.102 ± 0.009 | 0.209 ± 0.015 |
| 12 hours | 3.516 ± 0.039 | 0.878 ± 0.030 | 2.128 ± 0.055 | 0.416 ± 0.020 | 0.250 ± 0.014 | 0.298 ± 0.017 |
| 24 hours | 3.306 ± 0.041 | 1.025 ± 0.078 | 1.851 ± 0.058 | 0.904 ± 0.016 | 0.694 ± 0.036 | 0.452 ± 0.022 |
| 48 hours | 2.426 ± 0.023 | 0.826 ± 0.031 | 1.170 ± 0.042 | 0.878 ± 0.022 | 0.577 ± 0.029 | 0.991 ± 0.033 |
| 72 hours | 2.065 ± 0.032 | 0.717 ± 0.041 | 0.945 ± 0.052 | 0.619 ± 0.019 | 0.403 ± 0.009 | 1.057 ± 0.034 |
| 5 days | 1.824 ± 0.031 | 0.179 ± 0.024 | 0.408 ± 0.019 | 0.185 ± 0.016 | 0.108 ± 0.012 | 0.527 ± 0.023 |
After feeding larvae the leaves charged with ZnO NPs, zinc level was estimated quantitatively by Atomic Absorption Spectroscopy (AAS) in different tissues after specific time periods, and the values (mean ± SEM) are presented as μg of zinc ions per gram of insect tissue dry weight (or μg/ml of hemolymph). *Indicates that the values are below the detection limits of the instrument. #Indicates that the samples were not evaluated as the whole insect tissue sample, in case of control, yielded no signal.