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. 2020 Jan 31;10:1572. doi: 10.1038/s41598-020-58221-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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hUCB-MSCs reduced ROS levels induced by TGF-β1 in C2C12 cell myoblasts. (A) C2C12 myoblast cells were directly cocultured with hUCB-MSCs with or without TGF-β1. Cocultured myoblast cells were stained with H₂DCFDA to measure intracellular hydrogen peroxide using FACS caliber. (B) To confirm indirect effect of hUCB-MSCs, TGF-β1 treated C2C12 myoblast cells were cocultured with or without hUCB-MSCs and measured intracellular hydrogen peroxide levels. Graphs were plotted as the percentage relative to negative control and analyzed by one-way analysis. *P < 0.05, **P < 0.01, ***P < 0.001.