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. 2020 Jan 31;11:644. doi: 10.1038/s41467-020-14323-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a, b Histogram plot of read counts (top), scatter plot of reads counts vs. log2-transformed expression ratios (Log2R, bottom) and c, d cumulative frequency distribution (Cf) of Log2R of hepatic protein-coding mRNA (blue) and lncRNA (orange) expression changes. Data are from total RNA-Sequencing (RNA-Seq) in the liver of C57BL/6N mice after a, c HFD vs. (vs) CD feeding (n = 3 each) or b, d ad libitum feeding (AL) vs. 16 h of fasting (FA) (n = 4 each). e Log2R Cf of mRNA (blue) and lncRNA (orange) expression changes in the liver from lean (L) vs. obese (OB) patients without T2D (n = 4 per group). f Log2R Cf of mRNA (blue) and lncRNA (orange) expression changes in the liver of lean (L) vs. obese patients with T2D (n = 4 per group). g–j Quantitative reverse-transcription (RT) polymerase chain reaction (qPCR) for L-DIO-lncRNA expression in mice exposed to g HFD vs. CD feeding (n = 4–6). h Lepr^db vs. Dock^7m (n = 4). i AL vs. FA (n = 5 each) and j RF vs. FA (n = 4 each). c–f Statistical differences between mRNAs and lncRNAs were assessed using nonparametric Kolgomorov–Smirnov (KS) tests. p Values are given in the panels. Bar graphs represent mean ± s.e.m. with all data points plotted and differences in (g–j) were calculated using unpaired, two-tailed Student’s t tests. *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source Data file. Icons in a–f were created with BioRender.com.