Fig. 5.

In ovo quail cell injection, bead implantation in BA2 and whole mount immunostaining. Stages of development are indicated at the top of the panel. (A) Dorsal view of stage HH10 embryos injected with quail cells (arrowhead 1). (B) PBS or SDF-1 beads implantation into the BA2. Arrowheads 2 indicate the beads. (C) Second branchial arch region of chicken embryos injected with quail cells. SDF-1 bead attracts QCPN positive (quail) cells (C), whereas QCPN positive cells in the PBS beads treated embryos migrated normally and located in the central portion of the BA2 (yellow arrows in photo D).