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. 2020 Jan 31;10:1560. doi: 10.1038/s41598-020-58205-1

Table 1.

Characteristics of participants.

Control (n = 15) Diabetes without DR (n = 15) Diabetes with moderate to severe DR (n = 15) P value
Age (year) 52.4 (6.7) 53.7 (6.1) 56.8 (4.7) 0.116
Female 11 9 8 0.561
BMI (kg/m2) 34.3 (8.1) 32.9 (5.6) 30.0 (5.8) 0.204
Diabetes duration (yr) N/A 9.5 (6.7) 17.3 (2.3) 0.110
HbA1c (%) 5.4 (5.1, 5.6) 7.8 (6.7, 10.0) 8.8 (8.2, 10.8) 0.059d
Relative PIPR 0.36 (0.25, 0.41) 0.29 (0.19, 0.40) 0.10 (0.02. 0.21) <0.001b,c
Melatonin variable
aMT6s/Cr ratio (ng/mg) 11.3 (6.6, 22.3) 15.5 (3.5, 29.6) 1.2 (0.4, 3.9) <0.001b,c
DLMO presence 5/6 6/7 3/9 0.049
Sleep variables
Insomnia severity index 2 (1,7) 4 (2, 8) 10 (7, 14) 0.001b,c
Sleep duration (min) 396 (39) 397 (69) 387 (73) 0.895
Sleep efficiency (%) 84 (80.87) 86 (79, 89) 81 (69, 86) 0.134
Sleep duration variability (min) 65.6 (27.6) 55.4 (21.9) 89.3 (42.8) 0.023c
Mid-sleep time (hh:mm) 02:54 (01:06) 3:18 (01:18) 03:51 (01:06) 0.104
Rest-activity rhythm parameters
IV 0.78 (0.66, 0.84) 0.73 (0.57, 0.92) 0.77 (0.64, 0.88) 0.817
IS 0.48 (0.13) 0.43 (0.16) 0.45 (0.15) 0.608
M10-onset (h after midnight) 7.9 (6.1, 10.1) 8.1 (6.3, 10.7) 8.1 (6.9, 10.9) 0.694
L5-onset (h after noon) 11.8 (9.9, 12.2) 11.8 (11.1, 12.4) 12.7 (9.8, 13.7) 0.359
RA 0.88 (0.77, 0.93) 0.85 (0.75, 0.89) 0.77 (0.58, 0.85) 0.176
Sleep disordered breathing assessment
AHI (events/h)* 7.9 (1.8, 10.8) 12.0 (4.8, 31.5) 16.2 (11.6, 28.8) 0.041b
Neuropathy assessment
MNSI score 0 (0, 0) 1 (0, 3) 4.5 (1.5, 6.5) <0.001a,b,c
The LF/HF ratio** 177 (54, 313) 183 (78, 397) 190 (94, 380) 0.953
RMSSD 29.0 (16.0, 66.0) 12.5 (5.8, 24.3) 9.5 (4.3. 31.5) 0.025a,b

aSignificant differences between control vs T2D without DR; bsignificant differences between control vs T2D with DR, csignificant differences between T2D without DR and T2D with DR.

dComparison performed between T2D without DR and T2D with DR only.

*n = 15 for control participants, n = 14 for T2D without DR, and n = 12 for T2D with DR.

**n = 13 for control participants, n = 14 for T2D without DR, and n = 12 for T2D with DR.

AHI = apnea hypopnea index, BMI = body mass index, DLMO = dim light melatonin onset, IV = intradaily variability, IS = , interdaily stability, LF/HF ratio = low frequency/high-frequency ratio, MNSI = the Michigan Neuropathy Screening Instrument, RA = relative apmplitude, RMSSD = root mean square of the successive differences.