Supplementary Figure 6.

The combined treatment of CYT387 and trametinib synergistically inhibits the tumor cell signaling and induces apoptosis. A, L3.6pl cells were treated with CYT387 and trametinib as shown and Western blot was performed using indicated antibodies. B, Western blot analyses of mouse tissues were performed with indicated antibodies. C, Immunohistochemistry staining with pERK1/2, CD31, Ki67, and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay of the orthotopic tissues. D, Quantitative PCR with ErbB3 and IGF-1R primers was performed with RNA isolated from mouse tissues. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a reference.