FIGURE 3.

ARF6 is copolarized with RPH3A, RAB21, and PIP5K1C90 in Fb-stimulated neutrophils. (A–C) Copolarization of active ARF6 with RPH3A (A), RAB21 (B), and PIP5K1C90 (C) in neutrophils. Neutrophils attached to Fb-coated surfaces were stained with anti-active ARF6 and anti-RPH3A (A), anti-RAB21 (B), or anti-PIP5K1C90 (C), followed with Alexa 488 (green)– and Alexa 633 (red)–conjugated secondary Abs, respectively. Representative optical section images of two cells are shown. The quantification of colocalization is presented as Pearson correlation coefficient (Pearson Coefficient). Each data point represents a cell. The experiments were repeated three times. Scale bar, 3 μm. BF, bright field.