Table II. Flow cytometry mAb panel 2.
| Flow Panel 2 | |||||
|---|---|---|---|---|---|
| Spec. | Dye | μl | Supplier | Catalog No.; RRID | Clone |
| CD3 | PE | 1 | BioLegend | 12-0031-85; AB_465498 | 145-2C11 |
| CD4 | Allophycocyanin Cy7 | 1 | BioLegend | 100414; AB_312699 | GK1.5 |
| CD8 | PerCP Cy5.5 | 1 | BioLegend | 100734; AB_2075238 | 53-6.7 |
| CD44 | PE Cy7 | 1 | BioLegend | 103030; AB_830787 | IM7 |
| CD62L | BV421 | 1 | BioLegend | 104436; AB_2562560 | MEL-14 |
| CD103 | Allophycocyanin | 1.5 | BioLegend | 121414; AB_1227502 | 2E7 |
| CD69 | BV605 | 1 | BioLegend | 104530; AB_2563062 | H1.2F3 |
| CD49b | PE Dazzle 594 | 1 | BioLegend | 108924; AB_2565271 | DX5 |
Volume (μl) of Ab used per sample for panel 2 master mix.
RRID, Research Resource Identifier; Spec., specificity.