Table I. Summary for kinetic data obtained for human FcγRIIIB (CD16B) from SPR analysis.
| Protein | KD (M)a | RMAX | χ2 (RU2) |
|---|---|---|---|
| Irrelevant IgG1 | 1.34 × 10−5 | 102.6 | 0.425 |
| C309L/N563A/C575A | (1) 1.38 × 10−6 | 98.7 | 9.7 |
| (2) 2.23 × 10−6 | 113.9 | 8.39 | |
| D221N/C309L/N563A/C575A | (1) 1.53 × 10−6 | 80.6 | 3.7 |
| (2) 2.87 × 10−6 | 95.5 | 3.95 |
a
Proteins were run at the milligram per milliliter equivalent of the Fc control. Curves on each graph in Fig. 8 are approximate equivalent molar concentrations assuming a monomeric Fc. KD values are calculated assuming a monomeric Fc. Numbers in parentheses indicate experiment number.
RMAX, resonance maximum; RU, response units; SPR, surface plasmon resonance.