Table II. Summary for kinetic data obtained for human FcγRIIA167Arg (CD32A) from SPR analysis.
| Protein | KD (M)a | RMAX | χ2 (RU2) |
|---|---|---|---|
| Irrelevant IgG1 | 5.98 × 10−6 | 190.1 | 11.8 |
| C309L/N563A/C575A | (1) 6.20 × 10−7 | 131.9 | 13.3 |
| (2) 1.96 × 10−6 | 61.2 | 2.62 | |
| D221N/C309L/N563A/C575A | (1) 6.62 × 10−7 | 92.9 | 1.38 |
| (2) 1.75 × 10−6 | 34.1 | 0.49 |
a
Proteins were run at the milligram per milliliter equivalent of the Fc control. Curves on each graph in Fig. 8 are approximate equivalent molar concentrations assuming a monomeric Fc. KD values are calculated assuming a monomeric Fc. Numbers in parentheses indicate experiment number.
SPR, surface plasmon resonance.