Skip to main content
. 2019 Jul 24;33(10):11579–11594. doi: 10.1096/fj.201901178R

Figure 2.

Figure 2

Metabolic testing of Fgf13+/− mice and littermate controls housed at 22 or 30°C. Mice were placed in metabolic cages singly and allowed to acclimate, after which EE, food intake, and RER were measured. A–C) EE measured over 24 h. EE was compared and normalized to LBM via ANCOVA by first plotting those values against MRI-measured LBM (A). Data are plotted over time, with the dark cycle indicated by shading, (B) and as 24-h means with 2-way ANOVA used on 24-h sum data (C). D–F) Food intake measured over 24 h. Food intake was compared and normalized to LBM via ANCOVA by first plotting those values against MRI-measured LBM (D). Data are plotted over time, with the dark cycle indicated by shading, (E) and as 24-h means with 2-way ANOVA used on 24-h sum data (F). G) Body composition by MRI was measured at 13 wk of age, immediately after the end of the 24-h monitoring period. H, I) RER measured over 24 h. Data are plotted over time, with the dark cycle indicated by shading, (H) and as 24-h means with 2-way ANOVA used on 24-h sum data and on data for each individual mass type to assess effects of genotype, temperature, and interaction effects (I); n = 7–8/genotype for 22°C measurements and 11–13/genotype for 30°C measurements. NS, not significant. Comparisons between genotypes at each temperature were made using Holm-Sidak’s test for multiple comparisons. *P < 0.05.