Figure 7.

CNS- and hypothalamic-specific Fgf13 ablation recapitulates weight gain phenotype. A) Growth chart of Nes-Cre⁺;Fgf13^+/fl mice and Nes-Cre⁺ littermate controls housed at 30°C. Two-way repeated measures ANOVA was followed by Tukey’s test for multiple comparisons. B) Correlation of mouse weight and hypothalamic Fgf13 expression in Fgf13^+/− mice raised at thermoneutrality. C) Image of Cre-GFP expression in hypothalamus of Fgf13^fl/fl mouse. Scale bar, 500 µm. D) Weight gain of Cre-GFP and GFP injected Fgf13^fl/fl mice over 30 d. Two-way repeated measures ANOVA was followed by Tukey’s test for multiple comparisons; n = 5–6/genotype or injection condition. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; Het, heterozygous indicated significance. *P < 0.05, **P < 0.001.