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. 2020 Jan 24;13(1):dmm043166. doi: 10.1242/dmm.043166

Fig. 7.

Fig. 7.

HCT116 tumor-derived factors induce atrophy of C2C12 myotubes. (A) Co-culturing of C2C12 myotubes with HCT116 tumor cells for 48 h was performed, followed by assessment of myotube diameter (n=350-400). (B) Myotubes were stained with anti-MHC. (C) Representative western blotting and protein quantification for phosphorylated and total STAT3 (n=6). (D-F) Follow-up experiments were performed co-culturing HCT116 tumor cells and C2C12 myotubes with or without INCB018424 (400 nM). (D) Quantification of myotube diameter (n=350-400). (E) Myotubes were stained with anti-MHC. (F) Representative western blotting and protein quantification for phosphorylated and total STAT3 (n=3). Scale bars: 50 µm. Data are mean±s.d. **P<0.01, ***P<0.001, ****P<0.001 (Student's t-test: A-C; one-way ANOVA with Tukey's test: D-F).