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Flow-cytometric analysis of the read-through effect 48 h after treatment. Mean fluorescence intensity (MFI) in non-transfected cells and in those stably expressing the WT and the p.R50X constructs. Azithromycin and geneticin, 50 μg/ml; erythromycin, gentamycin and neomycin, 100 μg/ml; RTC13 and RTC14, 10 μM; Ataluren and amlexanox, 30 μM. Experiments were performed in duplicate for all conditions. Error bars indicate s.d. from duplicate experiments.
