Fig. 6.

Improved fusion of myoblasts in XMEA cells. (A) Representative images of control and patient cells immunostained for myosin heavy chain after 3 days of myotube differentiation with or without chloroquine treatment. Scale bars: 50 μm. (B) Fusion index calculated in control and patient cells, with and without chloroquine treatment. The fusion index was higher in the patient-derived cells than in the control cells, and treatment with chloroquine did not affect the percentage of nuclei in myotubes. We measured 45 fields for control differentiated myotubes, 35 fields for patient myotubes, and five fields for chloroquine-treated control and patient myotubes differentiated for 3 days. Three independent experiments were performed for untreated cells and one for chloroquine-treated cells. Magnification 200×, confocal microscopy. Green, myosin heavy chain; blue, DAPI. Data are presented as mean±s.d. ***P<0.001; ns, non-significant. Two-way ANOVA with Bonferroni post hoc for multiple comparisons.