Figure 5.

The effects of each compound on adipogenesis-related proteins, adiponectin, and GLUT4 levels in 3T3-L1 cells on day 8. Since all the same samples were used and meansurements were performed using 13 types of primary antibodies, only one β-actin image was shown. Protein levels were measured by electroblotting. B (Black): Undifferentiated cells without the addition of the MDI, C (Control): cells with the addition of the MDI, BER: cells with MDI and berberine (2.7 nM), and RO: cells with MDI and rosiglitazone (100 nM). The number with MC indicate the concentration (µM). Arrows indicate the addition of the MDI mixture. Data are presented as the mean ± SD from three independent experiments. The test results of the significance of protein expression levels are summarized in Table S2 of the Supplemetary Materials.