Fig. 3.

Wound-induced jasmonate signaling prevents stomatal-based cooling responses to elevated temperature and represses photosynthesis. (A) Average temperature of leaves from WT and jai1-1 plants grown for 17 d under CT and then transferred to CT or ET conditions for 5 d. Control plants (Con) received no wounding at the time of transfer. Two other sets of plants were challenged with caterpillar larvae (M. sexta) or daily mechanical wounding (wound) at the time of transfer. Leaf temperatures were measured with a thermal camera after allowing all plants (CT and ET treatments) to acclimate to room temperature (22 °C) for ∼1 h. (B and C) Stomatal conductance (B) and photosynthetic carbon assimilation (C) of WT and jai1-1 plants grown as described in A. Measurements were made on undamaged areas from damaged leaves using a LI-6800 portable photosynthesis system (LI-COR). Data points represent the mean ± SE of four biological replicates. Tables below A–C show P values from two-way ANOVA tests of the effect of leaf damage (D), temperature (T), and the interaction of D × T for each of the two genotypes tested (WT and jai1-1).