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. 2020 Jan 13;117(4):2014–2019. doi: 10.1073/pnas.1910722117

Fig. 1.

Fig. 1.

Silencing of Atox1 expression in MDA-231 breast cancer cells results in reduced cell migration velocity and Euclidean distance. Cell migration evaluation by 2D phase contrast video microscopy and single-cell tracking analysis (9-h video recorded between 30 and 48 h posttransfection, 1-min intervals) of MDA-231 cells with control or reduced Atox1 expression levels in standard CM or 5 µM Cu-supplemented CM (CM Cu). (A) Western blot analysis of Atox1 expression at 30 and 48 h post transfection with control (siCtrl) or Atox1-targeting siRNA (siAtox1). Beta-actin expression was determined as loading control. Error bars indicate SD of the mean (nExp = 3; statistics using Student’s t test). (B) Wind-rose plots show cell tracks (nExp = 3, nROI/Exp = 2, ntracked cells = 166, 170, 219, and 214 for siCtrl CM, siCtrl CM + Cu, siAtox1 CM, and siAtox1 CM + Cu, respectively). (C) Box-whisker plots present migration velocity and Euclidean distance of the tracked cells (nExp = 3, nROI/Exp = 2; Mann–Whitney U statistical test). The error bars indicate the minimum and maximum values for the data sets (thus, the entire range of data is shown in each case). *P < 0.05, **P < 0.01, ***P < 0.001.