Fig. 5.

Old trees lack senescence symptoms. (A–C) Heatmaps of changes in the expression of senescence-related transcription factors. The heatmaps are color coded by average expression of VC20, VC200, and VC600 (log₁₀FPKM). (D) Heatmaps of changes in the expression of miR164, miR319, miR156, and miR172. The heatmaps are color coded by expression ratios [log₂ (VC20_mean/VC20_mean), log₂ (VC200_mean/VC20_mean), log₂ (VC600_mean/VC20_mean)]; green, down-regulated miRNAs; orange, up-regulated miRNAs. (E) Boxplot of the expression (log₂ [FPKM]) of autophagy-related genes in VC20A, VC20B, VC20C, VC200A, VC200B, VC200C, VC600A, VC600B, and VC600C. Transcript levels of (F) WRKY57 and (G) NAC036 by qRT-PCR among more ages. The columns and error bars indicate the means and SDs (n = 3). The mean transcript levels of these genes are from trees of four age groups (3 and 5 y, 5Y; 20 and 34 y, 20Y; about 132, 152, 161, and 211 y, 200Y; and about 538, 553, and 667 y, 600Y).