Fig. 5.

The N terminus of CTCF is not sufficient to redirect cohesin to non-CTCF binding sites. (A) A schematic representations of WT CTCF and a chimeric construct with three AZF flanked by the N and C termini of CTCF and tagged with V5 at the C terminus. (B) De novo DNA-binding motifs identified with the indicated E-value for the top CTCF and AZF binding sites. (C, Left) Average profile of AZF-V5 (green) and RAD21(pink) occupancy at the 6,159 sites bound by AZF in mut CH12 cells. (Right) Average profile of CTCF (red) and RAD21 (pink) occupancy at the 6,159 sites randomly selected from the CTCF binding sites in mut CH12 cells. (D) Genome browser view of CTCF (red), RAD21 (pink), and AZF-V5 (green) occupancy in mut CH12 cells. The AZF binding sites are indicated by green arrows. The absence of RAD21 occupancy at AZF binding sites is indicated by green arrows.