Fig. 6.

H84T reduces influenza virus uncoating. MDCK cells were pretreated for 1 h with 0.1, 1, or 10 μM H84T, 10 μM D133G, or 40 μM of the fusion inhibitor ARB and infected with A/WSN/1933 (MOI 0.5) for 2.5 h at 37 °C. (A) Representative immunofluorescent micrographs of M1 antigen staining (green) and nuclei (blue) 2.5 h postinfection. Scale bars indicate 50 μm. (B) Micrographs of single cells from A, as denoted by the boxes in A. Scale bars indicate 5 μm. Data for A and B are representative of 15 independent experiments. (C) Quantitation of the number of cells with diffuse cytoplasmic M1 staining per field, as assessed by a trained observer in a blinded fashion. Statistical analyses were performed by t test. *P < 0.05 and **P < 0.01, as compared to the infected, untreated group. Error bars represent the SEM.