Fig. 5.

Immunohistochemistry for representative A1 and A2 astroglia markers in GFAP⁺ astroglia, and NOS2 and MHC class II molecules in Iba-1⁺ microglia at the acute phase of EAE. (A–D) Double immunostaining for (A) C3 (red) and GFAP (green), (B) S100A10 (red) and GFAP (green), (C) NOS2 (red) and Iba1 (green), and (D) MHC class II molecules (red) and IBa1 (green) in lumbar spinal cords from Cx47 fl/fl control (Upper) and Cx47 icKO (Lower) mice at the acute phase of EAE (dpi 17). (Scale bars: 100 µm.) (E–H) Quantitative analysis of (E) C3⁺ and (F) S100A10⁺ astroglia and (G) NOS2⁺ and (H) MHC class II⁺ microglia in lumbar spinal cords from Cx47 fl/fl and Cx47 icKO mice at the acute phase of EAE. All data are shown as means ± SEM of four mice per group. Significant differences were determined by an unpaired t test. *P < 0.05; **P < 0.01. n.s., not significant.