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. 2020 Jan 31;17:3. doi: 10.1186/s12950-020-0239-6

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — a Western blot assay of GLEA2 protein expression in DCs. DCs were transduced with Ad-GLEA2 or Ad-LacZ at an MOI of 200 for 24 h. The GLEA2 protein levels were analyzed by western blot assay. The GLEA2 protein could be detected after Ad-GLEA2 transduction. However, there was no expression of GLEA2 protein after Ad-LacZ transduction or in non-treated DCs. Lane 1, non-treated DCs; lane 2, DCs transduced with Ad-LacZ and lane 3, DCs transduced with Ad-GLEA2 b Western blot assay of GLEA2 protein expression in U251 cells. Lane 1, non-treated U251; lane 2, U251 transduced with Ad-LacZ shRNA and lane 3, U251 transduced with Ad-GLEA2 shRNA.