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. 2020 Jan 31;17:3. doi: 10.1186/s12950-020-0239-6

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — GLEA2-specific CTLs were tested for their ability to lyse U251 cells expressing GLEA2. a GLEA2-specific cytotoxic T lymphocytes (CTLs) were generated in vitro with 5 μg/ml of anti-CD3 antibody (clone 17A2; Biolegend) pre-bound to the culture plate and 2 μg/ml of soluble anti-CD28 antibody (clone 37.51; Biolegend) at 37 °C for 3ds. GLEA2-specific CTLs were tested against U251 cells. b GLEA2-specific cytotoxic T lymphocytes (CTLs) were generated by weekly stimulation of nonadherent peripheral blood lymphocytes with irradiated, autologous DCs transduced with Ad-GLEA2. GLEA2-specific CTLs were tested against U251 cells lacking GLEA2 expression. CTLs generated from Ad-LacZ transduced DCs and effector cells generated from T lymphocytes stimulated by PBS were used as controls. Experiments performed in triplicate showed consistent results.