Table 5.
Summary of Off-Target Analysisa
| ID | Sequence | Mismatch | Score | Chromosome position | Mutation |
|---|---|---|---|---|---|
| OT1 |
CAAAATTCAATAGACACATGGG -- hit GAATCTTCAATAGACACATNGG – query |
3 | 0.53 | Chr15:25520090-25,520,111 | Not detected |
| OT2 |
GTATATTCCATAGACACATGGG -- hit GAATCTTCAATAGACACATNGG – query |
3 | 0.86 | Chr4:109681139-109,681,160 | Not detected |
| OT3 |
TCATCTTCAAAAGACACATTGG -- hit GAATCTTCAATAGACACATNGG – query |
3 | 1.08 | Chr13:46227046-46,227,067 | Not detected |
| OT4 |
GTATCTTGAAAAGACACATGGG -- hit GAATCTTCAATAGACACATNGG – query |
3 | 1.3 | Chr11:87945053-87,945,074 | Not detected |
| OT5 |
GATTCTACAATGGACACATTGG -- hit GAATCTTCAATAGACACATNGG – query |
3 | 1.54 | Chr12:24874767-24,874,788 | Not detected |
| OT6 |
GAATTTCCAATGGACACATTGG -- hit GAATCTTCAATAGACACATNGG – query |
3 | 1.58 | Chr7:134494940-134,494,961 | Not detected |
| OT7 |
AAATCTTTAATACACACATCGG -- hit GAATCTTCAATAGACACATNGG – query |
3 | 1.78 | Chr4:107649971-107,649,992 | Not detected |
aMismatched bases are in bold