Fig. 4.

alpha diversity from third trimester of pregnancy to postpartum. Alpha diversity in pregnancy and postpartum as represented by observed richness (a), Shannon diversity (b) and Pielou evenness (c) based on samples from GDM (red, n = 43) and normoglycaemic (blue, n = 81) women with available faecal samples from 3rd trimester and 9 months postpartum. Samples were rarefied to an equal sequencing depth of 10,000 reads. Boxes represent interquartile range (IQR), with the inside line representing the median. Values within 1.5 × IQR of the first and third quartiles are represented by whiskers. Circles represent individual samples with lines connecting samples from the same individual. Students t-test were used to test differences between normoglycaemic and GDM pregnancies within each timepoint. Difference in richness, Shannon diversity and Pielou evenness between time points in GDM and normoglycaemic women combined was tested using a paired t-test