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. 2020 Jan 31;21:109. doi: 10.1186/s12864-020-6525-0

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 12 — Verification of differential expression of eight lipolytic genes using qRT-PCR analysis. Nutritional state is indicated by bar color, where green = fed, red = fasted and blue = refed condition. Values represent least-square means (LSM) and their standard error (LSE) of normalized expression of five cockerels (biological replicates) analyzed in duplicate. Expression levels, determined by qRT-PCR analysis, were normalized using the geNorm procedure in qBase software [59]. Values possessing different superscripts are significantly different as determined by analysis of variance (ANOVA) using the general linear models (GLM) procedure in Statistical Analysis System (SAS) software and with mean separation using Tukey’s Studentized Range Test