Fig. 6.

This gene network, identified in the D2FAST48h vs. D2REFED4h contrast, shows interactions between two transcription factors [nuclear receptor subfamily 3 group C member 1 (NR3C1) and peroxisome proliferator activated receptor delta (PPARD)] and their respective direct target genes (Panel a). This network was functionally annotated by IPA as “Endocrine Function” and “Lipid Metabolism”. Ingenuity Upstream Analysis predicts activation of NR3C1 (glucocorticoid receptor, GCR; Panel b), based on 18 up-regulated and 20 down-regulated AR-DEGs and slightly activated PPARD, based on the up-regulated state of 12 direct targets (red gene symbols) compared to 11 down-regulated genes (green gene symbols). Actually, the green gene symbols indicate higher expression in liver of D2REFED4h cockerels, while red gene symbols indicate higher expression in the D2FAST48h treatment group