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. 2020 Jan 31;18:26. doi: 10.1186/s12951-020-0582-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — The percentage of positive transfected cells (transfected Cells%, a), mean fluorescent intensity (MFI, b) and green fluorescence protein expression (c) without serum; relative percentage of transfection efficiency (d) and green fluorescence protein expression in the presence of serum (e) observed by fluorescence microscopy (4×). Data are displayed as mean ± SD (n = 3). *P < 0.05, compared with naked DNA, ^#P < 0.05, compared with CaP, ^&P < 0.05, compared with Lipofectamine-2000 (Lip-2000), ^@P < 0.05, compared with serum free group

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