Fig. 2.
SH-SY5Y-CRE cells can express from neuronal and ubiquitous promoters and can be transduced using different serotypes. A. SH-SY5Y cells (top panels) and SH-SY5Y-CRE cells (lower panels) infected by rAAVs express eGFP driven by different promoters. GFP driven by hSyn (4 days post infection), CAG (3 days post infection), CAMKIIa (5 days post infection) and EF1αc (4 days post infection) are shown. B. SH-SY5Y cells (top panels) and SH-SY5Y-CRE cells (lower panels) can be transduced by different rAAV serotypes. EF1αc-mCherry was packaged in rAAV1, rAAV2, rAAV5 and rAAV8 serotypes, delivered to cells, and imaged 4 days post infection. All images were taken at 20x magnification on an Olympus IX7 UV inverted microscope.