Figure 3.

MW library screening: gene knockdown level and Gal8 recruitment correlate. (A) Dose-dependent bioactivity for MW library. Overall p < 0.0001 for MW (59% of variation), dose (24%), and interaction (6%) by two-way ANOVA. For each dose, all polymers are statistically different from all other polymers by Tukey’s multiple comparisons test except within groups denoted “n.s.”. (B) Quantification of Gal8 recruitment confocal images reveals that Gal8 recruitment increases with increasing siRNA dose and increasing polymer MW. PBS-treated cells had near-zero response, highlighted with a dagger. Overall p < 0.0001 for MW (32% of variation), dose (35%), and interaction (25%). Single asterisks indicate polymers identified as significant hits by Dunnet’s comparison test. (C) Representative confocal micrographs of Gal8 recruitment at 50 nM siRNA dose. The images have been false-colored; white represents Hoechst staining; green represents Gal8; magenta represents automated annotation of Gal8 positive fluorescent spots.